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Long-read genome sequencing accelerated the cloning of <i>Pm69</i> by resolving the complexity of a rapidly evolving resistance gene cluster in wheat

2022-10-16

Abstract excerpt

Gene cloning in repeat-rich polyploid genomes remains challenging. Here we describe a strategy for overcoming major bottlenecks in the cloning of the powdery mildew (Pm) resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat (WEW). A conventional positional cloning approach encountered suppressed recombination due to structural variations, while chromosome sorting yielded an insufficient purity le...

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Literature Corpus work
b83e702f-5464-55b1-904f-03381d37b99c
DOI
10.1101/2022.10.14.512294
Open publication

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Long-read genome sequencing accelerated the cloning of <i>Pm69</i> by resolving the complexity of a rapidly evolving resistance gene cluster in wheatDOI 10.1101/2022.10.14.512294
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