Article

Beyond Agreement: Standardizing Crowdsourced Synapse Annotations through Proofreading in EM Connectomics

2025-09-27

Abstract excerpt

<h4> A bstract </h4> Reliable synapse identification in volumetric EM is hampered by subtle, 3D cues that yield variable human judgments. We present a standardized proofreading protocol that pairs explicit, operational criteria with machine-learning candidate generation and a two-stage calibration of annotators. In two larval Drosophila melanogaster volumes imaged at 8×8×8 nm, five raters (expert + 4 calibrate...

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Literature Corpus work: b2cfeb11-06a4-5dad-8f46-dfdd78032e4c
DOI: 10.1101/2025.09.26.678851

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