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Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)

1999-08-01

Abstract excerpt

Two sets of PCR primers were designed from DNA sequence which flanked the 3' end of the Pyrenopeziza brassicae mating type idiomorphs (MAT-1 and MAT-2). The first set of primers facilitated the amplification of a 750 bp product from genomic DNA of 50 isolates of P. brassicae, which included both mating types. The 750 bp PCR product was also amplified from DNA extracted from Brassica napus tissue infected with P. b...

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Literature Corpus work
b2376d2c-852b-5ef6-90ce-562e2bcefb6b
DOI
10.1006/pmpp.1999.0207
Open publication

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Development of PCR based diagnostic techniques for the two mating types of Pyrenopeziza brassicae (light leaf spot) on winter oilseed rape (Brassica napus ssp. oleifera)DOI 10.1006/pmpp.1999.0207
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