Article

Pathotyping of Newcastle disease virus: A novel single <i>BsaHI</i> digestion method of detection and differentiation of avirulent strains (lentogenic and mesogenic vaccine strains) from virulent virus

2021-07-19

Abstract excerpt

We provide a novel single restriction enzyme (RE) ( BsaHI ) digestion approach for detecting distinct pathotypes of the Newcastle disease virus (NDV). After scanning 4000 F gene nucleotide sequences in the NCBI database, a single RE ( BsaHI ) digesting site was discovered in the cleavage site. APMV-I “F gene” Class II specific primer-based reverse transcriptase PCR (RT-PCR) was utilized to amplify a 535 bp fragm...

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Literature Corpus work: ab9a549d-635c-553a-aa57-9b97e64f32c4
DOI: 10.1101/2021.07.16.452760

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Pathotyping of Newcastle disease virus: A novel single <i>BsaHI</i> digestion method of detection and differentiation of avirulent strains (lentogenic and mesogenic vaccine strains) from virulent virusDOI 10.1101/2021.07.16.452760Read

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