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Specific detection of biovars of Ralstonia solanacearum in plant tissues by nested-PCR-RFLP.

2000-03-01

Abstract excerpt

A sensitive and specific assay, based on a Nested-PCR-RFLP protocol, was developed for the detection of biovars of Ralstonia solanacearum, the causal agent of bacterial wilt. Oligonucleotide primer pairs were selected within the hrp gene region. Specific amplification of the hrp fragments was obtained for all R. solanacearum strains and also for two closely related species, Pseudomonas syzygii and the blood diseas...

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Literature Corpus work
a512e6c6-e94a-5f4f-8a5c-c604078a66d1
DOI
10.1023/a:1008742609761
Open publication

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Specific detection of biovars of Ralstonia solanacearum in plant tissues by nested-PCR-RFLP.DOI 10.1023/a:1008742609761
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