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Restriction fragment length polymorphism and sequence analysis of rRNA-ITS region of somatic hybrids produced between Pleurotus florida and Lentinula edodes

2016-03-01

Abstract excerpt

Nine pfle somatic hybrids and their parental strains Pleurotus florida and Lentinula edodes were characterized by restriction fragment length polymorphism (RFLP) and sequence analysis of ribosomal RNA internal transcribed spacer (rRNA-ITS) regions. The ITS1-5.8S-ITS2 region was amplified by primer pair ITS1 and ITS4. Restriction digestion of the amplified ITS1-5.8S-ITS2 region with AluI, HinfI, HpaII and HaeIII ge...

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Literature Corpus work
a261d398-7918-5bf5-92d0-b952339eebc6
DOI
10.1007/s13213-015-1121-2
Open publication

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Restriction fragment length polymorphism and sequence analysis of rRNA-ITS region of somatic hybrids produced between Pleurotus florida and Lentinula edodesDOI 10.1007/s13213-015-1121-2
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