Article

Application of an Rbd-Based Simple Immunoassay for Assessing Humoral Immunity against Emerging SARS-CoV-2 Virus Variants

2023-10-30

Abstract excerpt

We have developed a simple, rapid, high-throughput RBD-based ELISA to assess the humoral immunity against emerging SARS-CoV-2 virus variants. The cDNAs of the his-tagged RBD proteins of the virus variants were stably engineered into HEK cells secreting the protein into the supernatant, and RBD purification was performed by Ni-chromatography and buffer exchange by membrane filtration. The simplified assay uses sing...

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Literature Corpus work: 9fdbb4ef-f3f2-5a39-80eb-e0e4bd365ae9
DOI: 10.20944/preprints202310.1836.v1

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