Article

Detection of the pine wood nematode using a real-time PCR assay to target the DNA topoisomerase I gene

2010-05-01

Abstract excerpt

A species-specific real-time PCR assay targeting the DNA topoisomerase I gene has been developed to detect Bursaphelenchus xylophilus. The specificity of the assay was confirmed by the lack of amplification of genomic DNA from other Bursaphelenchus or Seinura species. The sensitivity test showed that the limit of the reaction was 0.01 ng of genomic DNA or one individual nematode, as small as an egg. The validity o...

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Literature Corpus work: 9c21b8f0-c2c0-54a5-8e5a-4be68697072d
DOI: 10.1007/s10658-009-9574-4

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