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Quantitative PCR coupled with melting curve analysis for rapid detection and quantification of Tenacibaculum maritimum in fish and environmental samples

2019-01-01

Abstract excerpt

The development of a rapid and accurate method for the diagnosis of tenacibaculosis caused by Tenacibaculum maritimum, is of great interest to improve the management and control of an outbreak of this fish disease. In this study, a real-time polymerase chain reaction assay coupled with melting curve analysis was designed for the detection and quantification of T. maritimum targeting a fragment of 164 bp of the seq...

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Literature Corpus work
904f8e64-2b26-570d-8e07-27a637d60abb
DOI
10.1016/j.aquaculture.2018.08.039
Open publication

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Quantitative PCR coupled with melting curve analysis for rapid detection and quantification of Tenacibaculum maritimum in fish and environmental samplesDOI 10.1016/j.aquaculture.2018.08.039
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