Article

Protocol for Safe, Affordable, and Reproducible Isolation and Quantitation of SARS-CoV-2 RNA from Wastewater

2021-02-17

Abstract excerpt

<h4>ABSTRACT</h4> The following protocol describes our workflow for processing wastewater with the goal of detecting the genetic signal of SARS-CoV-2. The steps include pasteurization, virus concentration, RNA extraction, and quantification by RT-qPCR. We include auxiliary steps that provide new users with tools and strategies that will help troubleshoot key steps in the process. This protocol is one of the safest...

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Literature Corpus work: 6e5b2b0e-f32a-574c-9984-04bf332876ca
DOI: 10.1101/2021.02.16.21251787

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