Article

Detection of Chalara fraxinea in common ash (Fraxinus excelsior) using real time PCR

2010-04-01

Abstract excerpt

A real time PCR assay was developed for the detection of Chalara fraxinea in common ash. PCR primers and Taqman probes, based on the internal transcribed spacer region of the multi-copy gene rDNA, were tested for specificity and sensitivity. The primers amplified an 81 bp fragment for C. fraxinea but did not amplify DNA from other Chalara species or from other fungi isolated from ash, whether pathogenic or saproph...

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Literature Corpus work: 4f185fcc-3e86-5a3b-818c-50aa02cbc616
DOI: 10.1111/j.1439-0329.2009.00610.x

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