Back to search

Article

Multiplex recombinase polymerase amplification (RPA) assay developed using unique genomic regions and coupled with a lateral flow device for rapid on-site detection of genus <i>Clavibacter</i> and <i>C. nebraskensis</i>

2020-08-22

Abstract excerpt

<h4>ABSTRACT</h4> Clavibacter is an agriculturally important bacterial genus comprising nine host-specific species/subspecies including C. nebraskensis ( Cn ), which causes Goss’s wilt and blight of maize. A robust, simple, and field-deployable method is required to specifically detect Cn in infected plants and distinguish it from other Clavibacter species for quarantine purposes and timely disease management...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
4ec9ddbd-4386-5063-99a8-5a1479d4ddff
DOI
10.1101/2020.08.22.262824
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Multiplex recombinase polymerase amplification (RPA) assay developed using unique genomic regions and coupled with a lateral flow device for rapid on-site detection of genus <i>Clavibacter</i> and <i>C. nebraskensis</i>DOI 10.1101/2020.08.22.262824
Synergetic effect of non-complementary 5’ AT-rich sequences on the development of a multiplex TaqMan real-time PCR for specific and robust detection of <i>Clavibacter michiganensis</i> and <i>C. michiganensis</i> subsp. <i>nebraskensis</i>Semantic proximity
Comparative genomics of the Gram-positive bacterial pathogen of maize, Clavibacter nebraskensis, reveals genomic variation between strains of varying aggressivenessSemantic proximity
Ecogenomic Diversity of <i>Clavibacter nebraskensis</i> in North AmericaSemantic proximity
Comparative genomics of strains of varying virulence of the Gram-positive bacterial pathogen of maize, Clavibacter nebraskensisSemantic proximity
A New Multiplex TaqMan qPCR for Precise Detection and Quantification of <i>Clavibacter michiganensis</i> in Seeds and Plant TissueSemantic proximity
Development and Validation of Genome-Informed and Multigene-based qPCR and LAMP Assays for Accurate Detection of <i>Dickeya solani</i> : A Critical Quarantine Pathogen Threatening Potato IndustrySemantic proximity
A genetic toolkit for investigating <i>Clavibacter</i> : markerless deletion, permissive site identification and an integrative plasmidSemantic proximity
Rapid Detection of <i>Burkholderia pseudomallei</i> with a Lateral Flow Recombinase Polymerase Amplification AssaySemantic proximity
Genetic diversity and population structure of Clavibacter michiganensis subsp. nebraskensis.Semantic proximity
Description of two novel non-pathogenic tomato-associated <i>Clavibacter</i> species: <i>Clavibacter seminis</i> sp. nov. and <i>Clavibacter quasicaliforniensis</i> sp. novSemantic proximity
“RSSC-Lineage Multiplex PCR” assay detects and differentiates Ralstonia solanacearum, R. pseudosolanacearum, R. syzygii and the R3bv2 subgroupSemantic proximity
Rapid and Sensitive Detection of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Tropical Race 4 Using a RPA-DETECTR AssaySemantic proximity
Select a neighboring publication to make it the new centre.