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Production of Polyclonal Antisera to the Coat Protein of Citrus Tristeza Virus Expressed in<i>Escherichia coli</i>: Application for Immunodiagnosis

1995-01-01

Abstract excerpt

Using specific primers based on the sequence of the Florida isolate T36 of citrus tristeza virus (CTV), the coat protein (CP) gene was amplified by RT-PCR (reverse transcription-polymerase chain reaction) from the severe California isolate SY568 of CTV. The RT-PCR product was cloned, sequenced, and subcloned into an expression vector pMAL-c2. The CTV CP was expressed as a fusion product containing a fragment of th...

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Literature Corpus work
3f66a638-af9c-5f3b-96cd-a83cbcf4eb9e
DOI
10.1094/phyto-85-691
Open publication

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Production of Polyclonal Antisera to the Coat Protein of Citrus Tristeza Virus Expressed in<i>Escherichia coli</i>: Application for ImmunodiagnosisDOI 10.1094/phyto-85-691
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