Back to search

Article

Detection of Xanthomonas citri subsp. citri from field samples using single-tube nested PCR

2011-06-01

Abstract excerpt

A single-tube nested PCR was developed for detection of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. The assay targets the pthA gene of Xcc and utilizes different annealing temperatures for the two primer pairs. It reliably detected as few as 1·0 × 10² Xcc cells, and was unaffected by the presence of PCR inhibitors. It was 10-fold and 8500-fold more sensitive than standard PCR a...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work
3d6917a7-faec-5955-8771-253444148bd3
DOI
10.1111/j.1365-3059.2010.02390.x
Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest
Interactive article relationship graphSelect a related publication card to move it into the centre and load its closest explainable connections. Solid lines are source-backed structured connections. Dashed lines are semantic discovery signals and are not scientific evidence.
Detection of Xanthomonas citri subsp. citri from field samples using single-tube nested PCRDOI 10.1111/j.1365-3059.2010.02390.x
Detection of Xanthomonas citri pv. viticola on grapevine by real-time PCR and BIO-PCR using primers designed from pathogenicity and xanthomonadin gene sequencesSemantic proximity
Ecological and Evolutionary Insights into Xanthomonas citri Pathovar Diversity.Semantic proximity
Multilocus sequence typing and whole genome single-nucleotide polymorphisms reveal phylogenetic diversity and population structure of Xanthomonas citri subsp. citri.Semantic proximity
The epidemiological significance of post-packinghouse survival of Xanthomonas citri subsp. citri for dissemination of Asiatic citrus canker via infected fruitSemantic proximity
Integrated Disease Management Strategies for Bacterial Citrus Cankers: Insights, Prospects and Future DirectionsSemantic proximity
Seasonal and post-harvest population dynamics of the Asiatic citrus canker pathogen Xanthomonascitri subsp. citri on grapefruit in FloridaSemantic proximity
Whole-Genome Analysis of a Historical Strain of Xanthomonas citri pv. citri Reveals Structural Variations.Semantic proximity
Evolution and spread of Xanthomonas citri subsp. citri in the São Paulo, Brazil, citrus belt inferred from 758 novel genomes.Semantic proximity
Insertion sequence- and tandem repeat-based genotyping techniques for Xanthomonas citri pv. mangiferaeindicae.Semantic proximity
Insights into comprehensive review of research on canker disease management for sustainable citrus productionSemantic proximity
Core genome MLST for epidemiological and evolutionary analyses of phytopathogenic <i>Xanthomonas citri</i>Semantic proximity
A MLVA genotyping scheme for global surveillance of the citrus pathogen Xanthomonas citri pv. citri suggests a worldwide geographical expansion of a single genetic lineage.Semantic proximity
Select a neighboring publication to make it the new centre.