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Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severity

2015-05-01

Abstract excerpt

Tumorigenic Agrobacterium-induced crown gall disease significantly limits the production of peach, cherry, and other fruit trees in China. Thus, the rapid detection of pathogens is necessary for disease prediction and management. In this study, we designed the primer pair ipt 3F/ipt 3R on the basis of the ipt gene. This primer pair effectively amplified a 247 bp DNA fragment from the transferred DNA (T-DNA) sequen...

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Literature Corpus work
30c488cf-c535-539c-bc1e-16da9696c345
DOI
10.1007/s10658-014-0586-3
Open publication

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Determination of tumorigenic Agrobacterium density in soil by real-time PCR assay and its effect on crown gall disease severityDOI 10.1007/s10658-014-0586-3
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