Article

Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniques

2023-10-31

Abstract excerpt

<title>Abstract</title> <p>Human milk microbiome studies are currently hindered by low milk bacterial/human cell ratios and often rely on 16S rRNA gene sequencing, which limits downstream analyses. Here, we aimed to find a method to study milk bacteria and assess bacterial sharing between maternal and infant microbiota. We tested four DNA isolation methods, two bacterial enrichment methods and three sequencing me...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2114a77e-1ff8-5143-af54-d0e3ad7eaf80
DOI: 10.21203/rs.3.rs-3513330/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniquesDOI 10.21203/rs.3.rs-3513330/v1Read

Analysis of microbial composition and sharing in low-biomass human milk samples: a comparison of DNA isolation and sequencing techniquesSemantic proximity Read

Optimization of metagenomic detection method for human breast milk microbiomeSemantic proximity Read

The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial ProfilesSemantic proximity Read

Evaluation of Methods to Optimise Shotgun Metagenomic Sequencing-Based Analysis of the Microbiome of MilkSemantic proximity Read

Metagenomic and bacterial culture studies of human breast milk microbiota: factors influencing its bacterial composition and diversitySemantic proximity Read

Characterization of the Diversity and Temporal Stability of Bacterial Communities in Human MilkSemantic proximity Read

Human milk: a source of more life than we imagineSemantic proximity Read

The impact of kit, environment and sampling contamination on the observed microbiome of bovine milkSemantic proximity Read

Got bacteria? The astounding, yet not-so-surprising, microbiome of human milkSemantic proximity Read

Metagenomic Analysis of Milk of Healthy and Mastitis-Suffering WomenSemantic proximity Read

Assembly of the infant gut microbiome and resistome are linked to bacterial strains in mother’s milkSemantic proximity Read

Breastfeeding patterns are associated with human milk microbiome composition: The Mother-Infant Microbiomes, Behavior, and Ecology Study (MIMBES)Semantic proximity Read