Article

Fast and reliable detection of doubled-haploids in Asparagus officinalis by stringent RAPD-PCR

2003-08-01

Abstract excerpt

A stringent RAPD-PCR protocol was developed for reliable distinction at an early stage between somatic and doubled-haploid androgenic regenerates emerging from asparagus anther culture by using decamer primers with a high G/C content and/or primers with a dinucleotide sequence. RAPD-PCR was used to test 87 putative doubled-haploids from seven donor plants of different varieties and breeding lines, with differing g...

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Literature Corpus work: 12fd7d2d-cc6a-56e5-babe-988ab732d2f5
DOI: 10.1017/s0021859603003447

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