Article

The expression of a baculovirus-derived chitinase gene increased resistance of tobacco cultivars to brown spot (Alternaria alternata).

2000-02-01

Abstract excerpt

The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) chitinase gene coding region was amplified using the polymerase chain reaction, inserted into a plasmid (pROK-2) and replicated in Escherichia coli XL1–blue. The recombinant plasmid was mobilised into Agrobacterium tumefaciens LBA 4404 and inoculated into tobacco leaf discs. The presence of the expressed chitinase in foliar tissue of kanamycin...

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Identifiers and source

Literature Corpus work: 0f606944-2c1d-5ed8-8c20-3ff34865fb9f
DOI: 10.1111/j.1744-7348.2000.tb00001.x

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