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Development of PCR-based Detection Methods for the Quarantine Phytopathogen Synchytrium endobioticum, Causal Agent of Potato Wart Disease

2005-09-01

Abstract excerpt

PCR-based methods were developed for the detection and quantification of the potato pathogen Synchytrium endobioticum in soil extracts and in planta. PCR primers, based on the internal transcribed spacer region of the multi-copy gene rDNA were tested for specificity, sensitivity and reproducibility in conventional and real-time PCR assays. Soil extraction procedures compared included the Hendrickx centrifugation (...

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Literature Corpus work
0b18bd1e-8b42-5b84-9622-1d699b8cbd61
DOI
10.1007/s10658-005-0297-x
Open publication

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