Article

Transcriptomic profiling of human skin biopsies in the clinical trial setting: A protocol for high quality RNA extraction from skin tumours

2018-04-23

Abstract excerpt

Transcriptomic profiling of skin disease using next generation sequencing allows for detailed information on aspects of RNA biology including gene expression, non-coding regulatory elements and gene splicing. The application of RNA sequencing to human skin disease and cancer is often hampered by degraded RNA. Here we describe a protocol that allows for consistently intact RNA to be extracted from snap frozen skin...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 09f9fca4-ba39-5ba0-a9d4-c4c7a0eab55f
DOI: 10.12688/wellcomeopenres.14360.1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transcriptomic profiling of human skin biopsies in the clinical trial setting: A protocol for high quality RNA extraction from skin tumoursDOI 10.12688/wellcomeopenres.14360.1Read

Feasibility of RNA Sequencing from Human Skin Microbiopsies in Actinic Keratosis and Basal Cell Carcinoma: A Paired Pilot Comparison with Shave BiopsySemantic proximity Read

Unravelling the landscape of skin cancer through single-cell transcriptomicsSemantic proximity Read

The Use of Single-Cell RNA-Sequencing and Spatial Transcriptomics in Understanding the Pathogenesis and Treatment of Skin DiseasesSemantic proximity Read

Challenges and Opportunities for the Translation of Single-Cell RNA Sequencing Technologies to DermatologySemantic proximity Read

Single-cell transcriptomics in dermatologySemantic proximity Read

Research Techniques Made Simple: Methodology and Clinical Applications of RNA Sequencing.Semantic proximity Read

Maximizing the Utility of Transcriptomics Data in Inflammatory Skin DiseasesSemantic proximity Read

Highly Efficient, Massively-Parallel Single-Cell RNA-Seq Reveals Cellular States and Molecular Features of Human Skin PathologySemantic proximity Read

An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin BiopsiesSemantic proximity Read

Research Techniques Made Simple: Whole-Transcriptome Sequencing by RNA-Seq for Diagnosis of Monogenic Disorders.Semantic proximity Read

RNA sequencing atopic dermatitis transcriptome profiling provides insights into novel disease mechanisms with potential therapeutic implicationsSemantic proximity Read

Benchmarking robust spatial transcriptomics approaches to capture the molecular landscape and pathological architecture of archived cancer tissuesSemantic proximity Read