Article

A simple PCR method for the detection of pathogenic spiroplasmas in crustaceans and environmental samples

2007-05-01

Abstract excerpt

A simple PCR method that uses a Chelex-100 extraction has been developed to detect the 16S rRNA gene of pathogenic spiroplasmas in commercially important crustaceans. The method detected spiroplasmas in the Chinese mitten crab Eriocheir sinensis, and the crayfish Procambarus clarki, as well as from environmental samples from host habitats. Compared with the Tissues/Cell Genomic DNA Isolation Kit method, the Chelex...

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Identifiers and source

Literature Corpus work: 0230f724-b241-54da-8bdd-534aa1e0139f
DOI: 10.1016/j.aquaculture.2007.01.032

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