Article
Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, Bangkok genotype.
The Southeast Asian journal of tropical medicine and public health - 1 Jun 1998
Attatippaholkun W H, Attatippaholkun M K, Nisalak A, Vaughn D W, Innis B L
Abstract excerpt
The nucleotide sequence of the nonstructural protein gene (1,610 bases) of dengue 3 virus (Bangkok genotype; CH53489 isolated in 1973) has been determined in both forward and reverse directions. The PCR based cycle sequencing technic by the enzymatic method of Sanger et al using a sequencing primer 5'-end labeled with gamma-32P-ATP was the method of our choice for sequence analysis. Two cDNA templates were...
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