Mutations that extend the specificity of the endonuclease activity of lambda terminase.

doi:10.1128/JB.181.1.218-224.1999

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Article

Mutations that extend the specificity of the endonuclease activity of lambda terminase.

Journal of bacteriology - 1 Jan 1999

Arens J S, Hang Q, Hwang Y, Tuma B, Max S, Feiss M

Abstract excerpt

Terminase, an enzyme encoded by the Nu1 and A genes of bacteriophage lambda, is crucial for packaging concatemeric DNA into virions. cosN, a 22-bp segment, is the site on the virus chromosome where terminase introduces staggered nicks to cut the concatemer to generate unit-length virion chromosom...

Topics

Amino Acid Substitution
Bacteriophage lambda
Chromosome Mapping
Endodeoxyribonucleases
Escherichia coli
Genes, Viral
Kinetics
Mutagenesis, Site-Directed
Mutation
Suppression, Genetic
Viral Plaque Assay

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