Analysis of a mutation affecting the specificity domain for prohead binding of the bacteriophage lambda terminase.

Abstract excerpt

Genetic studies have identified a specificity domain for prohead binding in the C-terminal 32 amino acids of gpA, the large subunit of bacteriophage lambda terminase (S. Frackman, D. A. Siegele, and M. Feiss, J. Mol. Biol. 180:283-300, 1984). In the present work, an amber mutation, Aam42, in the fifth-to-last codon of the A gene was found to be lethal in nonsuppressing hosts. The mutation, expected to generate...

Topics

• Bacteriophage lambda
• Base Sequence
• Codon
• Endodeoxyribonucleases
• Gene Expression Regulation, Viral
• Genetic Complementation Test
• Models, Biological
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• Virus Replication