Article
Chitobiase, a new reporter enzyme.
BioTechniques - 1 Dec 1998
Kalabat D Y, Froelich J M, Phuong T K, Forsyth R A, Newman V G, Zyskind J W
Abstract excerpt
N,N'-diacetylchitobiase (chitobiase) from the marine organism Vibrio harveyi is a highly stable reporter enzyme for gene fusions. This enzyme hydrolyzes the disaccharide chitobiose to N-acetyl glucosamine. The advantages of the reporter gene encoding chitobiase (chb) are: (i) that chitobiase and...
Topics
- Acetylglucosaminidase
- Amino Acid Sequence
- Bacterial Proteins
- Bacteriophages
- Base Sequence
- DNA Replication
- DNA-Binding Proteins
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Viral
- Genes, Reporter
- Genotype
- Lac Operon
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
