Article
Chitobiase, a new reporter enzyme.
BioTechniques - 1 Dec 1998
Kalabat D Y, Froelich J M, Phuong T K, Forsyth R A, Newman V G, Zyskind J W
Abstract excerpt
N,N'-diacetylchitobiase (chitobiase) from the marine organism Vibrio harveyi is a highly stable reporter enzyme for gene fusions. This enzyme hydrolyzes the disaccharide chitobiose to N-acetyl glucosamine. The advantages of the reporter gene encoding chitobiase (chb) are: (i) that chitobiase and N-acetyl-beta-D-glucosaminidase activities are missing in E. coli strains, (ii) chitobiase can be monitored using...
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