Article
A novel nozzle for more efficient sperm orientation to improve sorting efficiency of X and Y chromosome-bearing sperm
1 Dec 1998
Abstract excerpt
Efficient high-resolution detection of DNA for flow cytometric sorting of X and Y chromosome-bearing sperm is dependent on effectively orientating the sperm head to the laser beam in orthogonally configured flow systems. Normally, a beveled needle is required to enlarge the fraction of properly orientated sperm (flat side facing the laser beam). In this report, a modification to a standard jet-in-air nozzle for...
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