Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.

doi:10.1046/j.1432-1327.1998.2570630.x

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Article

Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe.

European journal of biochemistry - 1 Nov 1998

Yoko-o T, Roy S K, Jigami Y

Abstract excerpt

In the fission yeast Schizosaccharomyces pombe, gmh1+, gmh2+ and gmh3+ genes encode alpha-1,2-galactosyltransferase homologues. In an in vitro galactosyltransferase assay, the gmh3+ gene product showed galactose transfer activity toward a-methyl-D-mannoside as an acceptor. The disruption of gma12...

Topics

Base Sequence
DNA Primers
Galactose
Galactosyltransferases
Genes, Fungal
Kinetics
Phenotype
Schizosaccharomyces
Substrate Specificity

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