Article
An evaluation of RAPD fragment reproducibility and nature.
Molecular ecology - 1 Oct 1998
Pérez T, Albornoz J, Domínguez A
Abstract excerpt
Random amplified polymorphic DNA (RAPD) fragment reproducibility was assayed in three animal species: red deer (Cervus elaphus), wild boar (Sus scrofa) and fruit fly (Drosophila melanogaster). Ten 10-mer primers (Operon) were tested in two replicate reactions per individual under different stringency conditions (annealing temperatures of 35 degrees C or 45 degrees C). Two estimates were generated from the data:...
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