A cell-free translation-translocation system reconstituted with subcellular fractions from the wall-less variant fz;sg;os-1V of Neurospora crassa.

doi:10.1006/fgbi.1998.1066

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Article

A cell-free translation-translocation system reconstituted with subcellular fractions from the wall-less variant fz;sg;os-1V of Neurospora crassa.

Fungal genetics and biology : FG & B - 1 Aug 1998

Addison R

Abstract excerpt

A translation-translocation system reconstituted with subcellular fractions from the wall-less variant fz;sg;os-1V of Neurospora crassa reproduces in vitro translocation and processing of a secretory protein. The translation extract was isolated from the wall-less variant by gently lysing cells b...

Topics

Cell Wall
Cell-Free System
Centrifugation, Density Gradient
Endoplasmic Reticulum, Rough
Genetic Variation
Microsomes
Molecular Sequence Data
Neurospora crassa
Protein Biosynthesis
Translocation, Genetic

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