Article
(1,3)-beta-D-glucan synthase activity in mycelial and cell wall-less phenotypes of the fz, sg, os-1 ("slime") mutant strain of Neurospora crassa.
Experimental mycology - 1 Mar 1995
Polizeli M del L, Noventa-Jordão M A, da Silva M M, Jorge J A, Terenzi H F
Abstract excerpt
The cell wall-less fz, sg, os-1 ("slime") triple mutant of Neurospora crassa lacks (1,3)-beta-D-glucan synthase activity. fz, sg, os-1 segregants from slime x wild-type crosses initially germinate as a plasmodium (slime-like), but develop hyphae in a few hours and acquire a stable mycelial phenotype (mycelial intermediate). The cell wall-less phenotype (stable slime) can be reisolated from mycelial intermediates...
Topics
- Cell Fractionation
- Cell Wall
- Colony Count, Microbial
- Crosses, Genetic
- Genetic Complementation Test
- Glucosyltransferases
- Hexosamines
- Hexoses
- Membrane Proteins
- Membranes
- Mutation