Article
Mutational analysis of plasmid R64 thin pilus prepilin: the entire prepilin sequence is required for processing by type IV prepilin peptidase.
Journal of bacteriology - 1 Sept 1998
Horiuchi T, Komano T
Abstract excerpt
The thin pili of IncI1 plasmid R64, which is required for conjugation in liquid media, belong to the type IV pilus family. They consist of a major subunit, the pilS product, and a minor component, one of the seven pilV products. The pilS product is first synthesized as a 22-kDa prepilin, processe...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Coliphages
- Endopeptidases
- Escherichia coli
- Genes, Bacterial
- Genes, Dominant
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Protein Precursors
- Protein Processing, Post-Translational
- Sequence Deletion
- Transcription Factors