Article
Use of an amplified-fragment length polymorphism technique to fingerprint and differentiate isolates of Helicobacter pylori.
Journal of clinical microbiology - 1 Sept 1998
Gibson J R, Slater E, Xerry J, Tompkins D S, Owen R J
Abstract excerpt
Amplified-fragment length polymorphism (AFLP) analysis is the name given to a genotypic technique in which adapter oligonucleotides are ligated to restriction enzyme fragments and then used as target sites for primers in a PCR amplification process. The amplified fragments are electrophoretically separated to give strain-specific band profiles. We have developed a single-enzyme approach that did not require...
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