Article
Cloning of the Arabidopsis ent-kaurene oxidase gene GA3.
Proceedings of the National Academy of Sciences of the United States of America - 21 Jul 1998
Helliwell C A, Sheldon C C, Olive M R, Walker A R, Zeevaart J A, Peacock W J, Dennis E S
Abstract excerpt
The ga3 mutant of Arabidopsis is a gibberellin-responsive dwarf. We present data showing that the ga3-1 mutant is deficient in ent-kaurene oxidase activity, the first cytochrome P450-mediated step in the gibberellin biosynthetic pathway. By using a combination of conventional map-based cloning an...
Topics
- Amino Acid Sequence
- Arabidopsis
- Base Sequence
- Cloning, Molecular
- Cytochrome P-450 Enzyme System
- DNA, Recombinant
- Gene Expression Regulation
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Oxygenases