Article
Use of pulsed field gel electrophoresis to determine the source of microbial contamination of central venous catheters.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology - 1 Feb 1998
Livesley M A, Tebbs S E, Moss H A, Faroqui M H, Lambert P A, Elliott T S
Abstract excerpt
Microorganisms detected in situ on the distal tip of central venous catheters (CVC) within 90 min of insertion were investigated using pulsed-field gel electrophoresis to analyse genomic fragments obtained with the SmaI restriction enzyme. Thirty patients received a triple lumen CVC, which was inserted directly through the skin using the Seldinger technique. In a further 30 patients a triple lumen CVC was...
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