Functional implications of the 21-24 loop in recombinant prochymosin.

doi:10.1016/s0167-4838(98)00009-0

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Article

Functional implications of the 21-24 loop in recombinant prochymosin.

Biochimica et biophysica acta - 23 Apr 1998

Li H, Zhang Y, Dong Y, Yang K

Abstract excerpt

To investigate the role of the 21-24 (pepsin numbering) loop in prochymosin, the amino acid residues GTPP at positions 21 through 24 were replaced with GG, the equivalent loop residues from its homologous protein, penicillopepsin, or SG, GS by site-directed mutagenesis. The mutants except GTPP(21...

Topics

Animals
Chymosin
Circular Dichroism
Enzyme Precursors
Escherichia coli
Humans
Mice
Mutagenesis, Site-Directed
Mutation
Protein Folding
Rats
Recombinant Proteins
Structure-Activity Relationship

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