Article
[Fluorescence-based semi-automated gene scan with microsatellite markers by multiplex PCR techniques].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics - 10 Apr 1998
Kuang S, Wang J, Huang W, Zhang Y, Lu L, Cheng Z, Jin L
Abstract excerpt
OBJECTIVE: To develop a high output and low cost multiplex PCR approach to facilitating large scale gene scan and gene typing with microsatellite markers. METHODS: 5-15 pairs of primers of microsatellite loci were added in one single tube with 5ul reaction volume, containing 10 mmol/L Tris-HCl (p...
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