Attenuation of recombinant vesicular stomatitis viruses encoding mutant glycoproteins demonstrate a critical role for maintaining a high pH threshold for membrane fusion in viral fitness.

Abstract excerpt

A plasmid-based recovery system was used to generate four unique vesicular stomatitis virus (VSV) mutants that encode glycoproteins (G proteins) with single or double amino acid substitutions in two conserved acidic residues adjacent to the putative G protein fusion domain. Previously we demonstr...

Topics

• Animals
• Antiviral Agents
• Binding, Competitive
• Cell Line
• Chloroquine
• Cricetinae
• HeLa Cells
• Humans
• Hydrogen-Ion Concentration
• Membrane Fusion
• Mutation
• Vesicular stomatitis Indiana virus
• Viral Envelope Proteins
• Viral Fusion Proteins