Article
Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific M. bovis pncA sequence polymorphism.
Journal of clinical microbiology - 1 Jan 1998
Espinosa de los Monteros L E, Galán J C, Gutiérrez M, Samper S, García Marín J F, Martín C, Domínguez L, de Rafael L, Baquero F, Gómez-Mampaso E, Blázquez J
Abstract excerpt
An allele-specific amplification method based on two genetic polymorphisms to differentiate Mycobacterium tuberculosis from Mycobacterium bovis was tested. Based on the differences found at position 169 in the pncA genes from M. tuberculosis and M. bovis, a PCR system which was able to differentiate most of the 237 M. tuberculosis complex isolates tested in one of the two species was developed. All 121 M....
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