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Article

Amino Acids in Maturation Medium and Presence of Cumulus Cells at Fertilization Promote Male Pronuclear Formation in Porcine Oocytes Matured and Penetrated in Vitro1

1 Dec 1997

Abstract excerpt

The present study was conducted to examine the ability of porcine oocytes to achieve male pronuclear (MPN) formation when they are matured and penetrated in vitro under various culture conditions. When cumulus-enclosed oocytes were cultured for 24-48 h in modified Whitten's medium (pH 7.4) supplemented with 10% porcine follicular fluid, 10 IU eCG/ml, and 10 IU hCG/ml (designated mWM-FG), nuclear maturation of...

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PMID: 9408257
DOI: 10.1095/biolreprod57.6.1478

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