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Fertilization and Subsequent Development in Vitro of Pig Oocytes Inseminated in a Modified Tris-buffered Medium with Frozen-Thawed Ejaculated Spermatozoa1

1 Oct 1997

Abstract excerpt

The present study examined the penetrability of pig oocytes by frozen-thawed ejaculated boar spermatozoa, prepared by the pellet method, coincubated in a modified Tris-buffered medium. Subsequent embryonic development of fertilized oocytes was also determined. Porcine oocyte-cumulus complexes were cultured in BSA-free North Carolina State University (NCSU) 23 medium containing porcine follicular fluid (10%),...

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PMID: 9314573
DOI: 10.1095/biolreprod57.4.729

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