Article
Transcriptional regulation by HNF-4 of the steroid 15alpha-hydroxylase P450 (Cyp2a-4) gene in mouse liver.
The Journal of steroid biochemistry and molecular biology - 1 Jul 1997
Yokomori N, Nishio K, Aida K, Negishi M
Abstract excerpt
The mouse P450 gene Cyp2a-4 encodes the hepatic steroid 15apha-hydroxylase. We have defined in the 5'-flanking sequence of Cyp2a-4 gene, a composite regulatory element (-61AGACCAAAGTCCGGCCTTC-42) which contains a potential CpG methylation site at position -50. Gel-shift assays indicate that this...
Topics
- Animals
- Aryl Hydrocarbon Hydroxylases
- Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
- Cytochrome P-450 Enzyme System
- DNA Footprinting
- DNA Methylation
- DNA-Binding Proteins
- Female
- Genes, Reporter
- Hepatocyte Nuclear Factor 4
- Liver
- Male
- Mice
- Mutation
- Phosphoproteins
- Promoter Regions, Genetic
- Steroid Hydroxylases
- Transcription Factors