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Application of a nested, multiplex PCR to psittacosis outbreaks

1 Aug 1997

Abstract excerpt

We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene. The first-step PCR was genus specific, and the second-step PCR was multiplexed (i.e., had multiple primer sets in the same tube) and...

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PMID: 9230378
DOI: 10.1128/jcm.35.8.2043-2046.1997

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