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Article

Identification of genes with specific expression in pancreatic cancer by cDNA representational difference analysis

1 Jun 1997

Abstract excerpt

cDNA representational difference analysis (cDNA-RDA) is a polymerase-chain-reaction-coupled subtractive and kinetic enrichment procedure for the isolation of differentially expressed genes. In this study, the technique was used to isolate novel genes specifically expressed in pancreatic cancer. cDNA-RDA was done on cDNA reverse transcribed from a poly(A)+ mRNA pool made from 10 cancer tissues (tester) by using as...

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Share to Advanced biosensing and bioanalysis techniques
Share to Genomic variations and chromosomal abnormalities
Share to Pancreatic and Hepatic Oncology Research

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PMID: 9172000
DOI: 10.1002/(sici)1098-2264(199706)19:2<97::aid-gcc5>3.0.co;2-v

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