Article
Use of polymerase chain reaction-based single strand conformational polymorphism and denaturing gradient gel electrophoresis methods for detection of sequence variation of ribosomal DNA of Trypanosoma cruzi.
International journal for parasitology - 1 Mar 1997
Stothard J R, Frame I A, Miles M A
Abstract excerpt
Polymerase chain reaction (PCR) was used to amplify the V1 region of the small sub-unit (18S) ribosomal DNA gene from representative strains of Trypanosoma cruzi. In order to screen for sequence variation, amplification products were subsequently analysed by single strand conformational polymorph...
Topics
- Animals
- Base Sequence
- DNA Primers
- DNA, Protozoan
- DNA, Ribosomal
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Humans
- Molecular Sequence Data
- Parasitology
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Trypanosoma cruzi