Article
Specific mutation near the primary donor in photosystem I from Chlamydomonas reinhardtii alters the trapping time and spectroscopic properties of P700.
Biochemistry - 11 Mar 1997
Melkozernov A N, Su H, Lin S, Bingham S, Webber A N, Blankenship R E
Abstract excerpt
Time-resolved absorption and fluorescence spectroscopy were used to investigate the energy and electron transfer processes in the detergent-isolated photosystem I core particles from the site-directed mutant of Chlamydomonas reinhardtii with the histidine-656 of PsaB replaced by asparagine [HN(B656) mutation]. The specific mutation near the primary donor molecule results in a 40 mV increase in the P700/P700+...
Topics
- Animals
- Chlamydomonas reinhardtii
- Chlorophyll
- Electrochemistry
- Electron Transport
- Kinetics
- Light-Harvesting Protein Complexes
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Photosynthetic Reaction Center Complex Proteins
- Photosystem I Protein Complex