Article
Influence of the axial ligands on the spectral properties of P700 of photosystem I: a study of site-directed mutants.
Biochemistry - 24 Oct 2000
Krabben L, Schlodder E, Jordan R, Carbonera D, Giacometti G, Lee H, Webber A N, Lubitz W
Abstract excerpt
Two histidines provide the axial ligands of the two chlorophyll a (Chl a) molecules which form the primary electron donor (P700) of photosystem I (PSI). Histidine 676 in the protein subunit PsaA, His(A676), and histidine 656 in subunit PsaB, His(B656), were replaced in the green algae Chlamydomnas reinhardtii by site-directed mutagenesis with nonpolar, uncharged polar, acidic, and basic amino acid residues. Only...
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