Article
An improved method for MN genotyping by the polymerase chain reaction.
International journal of legal medicine - 1 Jan 1996
Nakayashiki N, Sasaki Y
Abstract excerpt
A novel method of human MN blood group genotyping is reported using the polymerase chain reaction. Genotyping is based on two base substitutions characteristic of M and N alleles in the 2nd exon of the glycophorin A gene. Using a newly designed primer trio, PCR products for M (255 bp) and N (270 bp) alleles are rapidly and simultaneously detected by a single PCR procedure and subsequent polyacrylamide gel...
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