Article
[Ultrafast freezing of the embryos of outbred and inbred mouse strains].
Ontogenez - 1 Jan 2000
Krivokharchenko A S, Serobian G A, Shakhbazian A K, Sadovnikov V B
Abstract excerpt
Survival of 8-cell mouse embryos of inbred strains C57B1 and DBA and outbred strain NMRI was studied after ultrafast freezing. The embryos were placed in 10% glycerol for 10 min, transferred in plastic tubes filled with a mixture of glycerol and 1 M sucrose (3:7), and immersed in liquid nitrogen within 1.5 min. The tubes were thawed in air at the room temperature, the embryos were washed in 0.5 M sucrose and...
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