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Polymerase Chain Reaction Species Diagnostic Assay for Anopheles quadrimaculatus Cryptic Species (Diptera: Culicidae) Based on Ribosomal DNA ITS2 Sequences

1 Jan 1996

Abstract excerpt

Species-specific differences in the nucleotide sequences of the 2nd internal transcribed spacer (ITS2) of nuclear ribosomal DNA (rDNA) were used to develop a diagnostic assay based on the polymerase chain reaction (PCR) that can distinguish 4 of the 5 cryptic sibling species in the common malaria mosquito, Anopheles quadrimaculatus Say, complex. The assay requires only a small amount of tissue from an individual...

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PMID: 8906913
DOI: 10.1093/jmedent/33.1.109

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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