Article
Development of gene transfer methods for Rubrivivax gelatinosus S1: construction, characterization and complementation of a puf operon deletion strain.
Molecular & general genetics : MGG - 25 Sept 1996
Ouchane S, Picaud M, Reiss-Husson F, Vernotte C, Astier C
Abstract excerpt
Gene transfer systems were developed in Rubrivivax (Rx.) gelatinosus S1. First, a system for conjugative transfer of mobilizable plasmids from Escherichia coli to Rx. gelatinosus S1 was established. Secondly, optimal conditions for the transformation of Rx. gelatinosus S1 by electroporation were...
Topics
- Bacterial Proteins
- DNA Transposable Elements
- Electroporation
- Escherichia coli
- Gene Deletion
- Gene Expression Regulation, Bacterial
- Gene Transfer Techniques
- Genetic Complementation Test
- Light-Harvesting Protein Complexes
- Phenotype